Research Articles

RP-HPLC Method Development and Validation for the simultaneous estimation of Atazanavir sulphate and Ritonavir in bulk and tablet dosage form

Vol. 1 No. 3 (2014) 50-54 Published October 27, 2014

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Abstract

A simple, robust, precise and accurate reverse phase liquid chromatographic method was developed for simultaneous estimation of Atazanavir sulphate and Ritonavir in combined tablet dosage form by RP-HPLC method. Chromatography was carried out on a Nucleodur C18 column (150 mm x 4.6 mm x 5 m) using Acetonitrile: Methanol: Phosphate buffer, pH was adjusted to 3.0 with orthophosphoric acid in the ratio of 44:11:45 (V/V) as a mobile phase at a flow rate of 1.5 mL min-1 and eluents were monitored at 210 nm. The calibration curves were linear over the range of 34 102 ?g mL-1 for Atazanavir sulphate and 10 30 ?g mL-1 for Ritonavir. The average retention time of Atazanavir sulphate and Ritonavir was found to be 3.133 min and 6.133 min respectively. The method was reproducible, with good resolution of Atazanavir sulphate and Ritonavir. The results of the analysis have been validated statistically.

References

  1. Nilesh, A. B.; Shailendra, P. K.; Shailesh, N. S.; Saroj, V. S.; Vishnu, P. C., Spectrophotometric simultaneous determination of Atazanavir and Ritonavir in combined tablet dosage form by ratio derivative and area under curve method. Der. Pharma. Chemica. 2012, 4(01), 208-213.
  2. Nanda, R. K.; Kulkarni, A. A.; Yadav, P. B., Simultaneous spectrophotometric estimation of Atazanavir sulfate and Ritonavir in tablet. Der. Pharma. Chemica. 2011, 3(03), 84-88.
  3. Nagaraju, P. T.; Sreekanthreddy, K. P.; Chinnaganganna, P.; Venugopal, N.; Ravindranath, S.; BhanuPriya, B.; Satyanandam, S., RP-HPLC method for determination of Ritonavir in pharmaceutical preparations. Int. J.Pharm. Sci. Rev. Res. 2012, 13(2), 55-57.
  4. Arun, R.; Anton, A. S., Development and Validation of Analytical method for Lopinavir and Ritonavir by HPLC. Int. J. Drug Dev. Res. 2013, 5(2), 1166-1171.
  5. Dario, C.; Franco, M.; Diego, R.; Norberto, P., Determination of Atazanavir in Human Plasma by High-Performance Liquid Chromatography with UV Detection. J. Chromatogr. Sci. 2008, 46, 485-488. http://dx.doi.org/10.1093/chromsci/46.6.485 DOI: https://doi.org/10.1093/chromsci/46.6.485
  6. Salinthip, J.; Kittisak, S.; Hutimamatayat, S. K.; Prapin, W., Simultaneous determination of plasma Lopinavir and Ritonavir by chemometrics- assisted spectrophotometry and comparisonwith HPLC method. Maejo Int. J. Sci. Tech. 2013, 7(02), 248-257.
  7. Anusha, T.; Ashwini, G.; Annapurna, R. C.; Aravind, S.; Abbarajuprasana, L.;Avinash, K., Method development and validation for the simultaneous estimation of Atazanavir and Ritonavir in pharmaceutical dosage form by RP-HPLC. Int. J. Pharm. Chem. Bio. Sci. 2012, 03 (01), 44-54.
  8. Swetha M. A.; Ravindrareddy,Y., Method development and validation of Atazanavir and Ritonavir in a combined dosage form By RP-HPLC Method. Int. J. Pharm. Tech. 2011, 3(03), 3316-3334.
  9. Nilesh, A. B.; Shailendra, P. K.; Shailesh, N. S.; Saroj, V. S.; Vishnu, P. C., Spectrophotometric simultaneous determination of Atazanavir and Ritonavir in combined tablet dosage form by ratio derivative and area under curve method. Der. Pharma. Chemica. 2012, 4(01), 208-213.
  10. Sathish, K, K.; Sujana, K.; Prameela, R. A., New validated RP-HPLC method for the determination of Atazanavir Sulphate in bulk and dosage form. Der. Pharma. Chemica. 2012, 4(03), 1305-1310.
  11. Srinivasu , K.; Venkateswara, R. J.; Appala, R.N.; Mukkanti, K. A.,Validated RP- HPLC method for the determination of Atazanavir in pharmaceutical dosage form. J. Chem. 2011, 8(01), 453-456. http://dx.doi.org/10.1155/2011/812879 DOI: https://doi.org/10.1155/2011/812879
  12. Manoj, G.; Anil, B.; Bhanubhai, S.; Ishwarsinh, R.; Urvish, D.; Arpit, P.; Ketan,V., Simultaneous Determination of Ritonavir and Atazanavir in combined tablet dosage form by HPTLC. Asian. J. Biomed. Pharm. Sci. 2012, 2(15), 15-19.
  13. ICH Q8 (R2), Pharmaceutical Development, 2009.
  14. ICH Q2 (R1), Validation of analytical procedures: Text and methodology, 2005, Geneva, Switzerland.

Author information

  • Ananda Thangadurai Subramaniam

    Swamy Vivekanandha College of Pharmacy, The T.N.M.G.R Medical University, Chennai

  • Jambulingam Munusamy

    Swamy Vivekanandha College of Pharmacy, The T.N.M.G.R Medical University, Chennai

  • Saravanakumar Sengodan

    Swamy Vivekanandha College of Pharmacy, The T.N.M.G.R Medical University, Chennai

  • Kamalakannan Danapal

    Swamy Vivekanandha College of Pharmacy, The T.N.M.G.R Medical University, Chennai

  • Haritha D Siva Ganga Lakshmi

    Swamy Vivekanandha College of Pharmacy, The T.N.M.G.R Medical University, Chennai

  • Varatha S Kunan

    Swamy Vivekanandha College of Pharmacy, The T.N.M.G.R Medical University, Chennai

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Subramaniam, A. T.; Munusamy, J.; Sengodan, S.; Danapal, K.; Siva Ganga Lakshmi, H. D.; Kunan, V. S. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Atazanavir Sulphate and Ritonavir in Bulk and Tablet Dosage Form. J Pharm Chem 2014, 1 (3), 50-54. https://doi.org/10.14805/jphchem.2014.art19.
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Received
July 20, 2014
Published
October 27, 2014
Section
Research Articles
Pages
50-54

Keywords

  • Atazanavir Sulphate
  • Ritonavir
  • RP-HPLC
  • Validation
  • Acetonitrile
  • Methanol

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