#57 Specific and rapid detection of Mycobacterium tuberculosis in metal complexes by RT-PCR method

Abstract

Tuberculosis was a major public health problem and highly prevalent in India. The treatment of tuberculosis is becoming more challenging; it requires accurate and early diagnosis, screening of drug resistance, and effective directly observed therapy and short course. Metal complexes are already in clinical use and encourage further studies for new metallodrugs in the treatment of tuberculosis. With the extensive investigations on metal complexes derivatives on its anti-microbial and anti-tubercular activities, a new series of metal complexes were synthesized and tested against Mycobacterium tuberculosis H37Rv strain by using RT-PCR method. Salicylaldehyde (0.1 mM) was dissolved in methanol (10 mL) was added to L-Alanine (0.1 mM) followed by 2 mL of glacial acetic acid. The reaction mixture was heated under reflux for 6 h with continuous stirring. The product obtained was filtered, washed with methanol and ether. The Schiff base obtained was dissolved (0.2 g, 0.33 mM) in a mixture of ethanol-water heated to the boiling point was added to an alcoholic solution of the various metal chlorides, and refluxed for 1 h for complete precipitation. The products were filtered off, washed with ethanol and water. The synthesized metal complexes were evaluated for M. tuberculosis H37Rv strain by using RT-PCR method. Amplification of three target sequences from unrelated genes, namely, hsp 65 (165 bp), dnaJ (365 bp), and insertion element S 6110 (541 bp) by PCR was carried out in synthesized metal complexes and isoniazid as control. Cobalt and Zinc containing metal complexes were found to potent against the mycobacterium tuberculosis and the amplication in the RT-PCR found to be absent /negative. RT-PCR method presented as fast and precise to detect Mycobacterium tuberculosis. The synthesized metal complexes containing cobalt and zinc may be found to be a potent drug of choice for the treatment of tuberculosis.

Fig. Reaction