Research Articles
Selvakumar Duraipandi, Vijaya Selvakumar
The guggulu containing polyherbal formulations in Ayurveda have been used for treating various inflammatory conditions. A simple HPTLC method has been developed to qualitatively analyze the formulations that claimed to have contained guggulu using the Guggulsterones isolated from the guggulu raw material as a marker. The isolation of Guggulsterones from the resinous gum obtained from the plant was used as a marker to determine the Guggulsterone content in the formulations. The study showed that all the preparations taken for analysis that claimed to contain Guggulsterones was originally having the contents but in variable amounts depending on the amount of resin taken for the preparation. Due to the fact that Guggulsterones are very expensive marker compounds if procured separately as Guggulsterone E and Z forms, this method can be used for routine qualitative analysis of presence of Guggulsterones as an in-house quality control method.
1-4
Umasankar Kulandaivelu, Bhawatha Chawada, Shireesha Boyapati, Alavala Rajasekhar Reddy
Arylalkylidene derivatives of aminotriazoles ( 3a-3j ) were synthesized and tested for their antimicrobial and anticancer activity. Four non-pathogenic bacteria [ E. coli  (NCIM 2068), K. pneumoniae  (NCIM 2957), S. aureus  (NCIM 2079), B. subtilis  (NCIM 2921)] two fungi [ C. albicans , A. niger ] and two cancer cell lines [HBL-100 and HT-29] were employed in the study. All the compounds were found to have better antibacterial activity against B. subtilis  than Ciprofloxacin (standard) and compound 3i  was equivalent to Ciprofloxacin in inhibiting S. aureas . Similarly all the compounds inhibited the growth of A. niger  better than Fluconazole and compound 3c  was equivalent to Fluconazole (standard) in inhibiting C. albicans . In case of anticancer activity none of the molecule exhibited activity better than the standard used (Methotrexate), though they have inhibitory concentration at submicromolar level.
5-9
Harini Kanchukommula, Ananda Thangadurai Subramaniam, Jambulingam Munusamy, Kamalakannan Danapal, Saravanakumar Sengodan
A RPHPLC chromatographic method was developed and validated for the determination of Quinapril and Hydrochlorothiazide in bulk powder and in pharmaceutical formulations. Quinapril and Hydrochlorothiazide can be separated on Zorbax Eclipse XDB, C18 column (150 x 4.6 mm, 5 mm) at 30 C using Acetonitrile: Phosphate buffer, pH 4.5 was adjusted with o-phosphoric acid in the ratio of 35:65 v/v as a mobile phase at flow rate of 0.9 mL min -1Â and detected at 210 nm. The retention time of Quinapril and Hydrochlorthiazide was found to be 2.099 min and 5.537 min respectively. The validation of the proposed method was carried out for specificity, linearity, accuracy, precision, LOD, LOQ and robustness. Calibration was linear over a range of 50-300 g mL -1 Â and 31.25187.5 g mL -1Â with correlation coefficient of 0.999 for Quinapril and Hydrochlorthiazide, respectively. The robustness of the method was evaluated by deliberately altering the chromatographic conditions. The method developed can be applicable for quality control analysis.
10-14
Rajesh A Keraliya, Madhabhai M Patel
A method for the development of press coated tablet of atenolol for pulsatile delivery was investigated for chronotherapy of hypertension. Effect of viscosity of Hydroxypropylcellulose (HPC) on pulsatile release of atenlol was studied by press coating atenolol core tablet using different viscosity grade HPC and varying coat weight. L-HPC, M-HPC and H-HPC viscosity garde with 75, 100 and 150 mg coat weight were press coated over atenolol core tablets to delay release of atenolol. The batches, HP1-HP9, exhibited an increase in lag time in response to increase in viscosity and coat weight. Two of the batches HP5 and HP7 have shown a burst release of atenolol after 6.5 and 6.0 h lag time respectively, which is suitable for pulsatile drug delivery of atenolol for chronotherapy of hypertension.
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