#172 A novel instrumental method for simultaneous estimation of cardiovascular drugs

Abstract

The market is flooded with a combination of drugs in various dosage forms. Multi-component formulations are in demand due to greater patient acceptability, increased potency, multiple actions, fewer side effects, and quicker relief. Analytical methods are needed to analyze multi-component formulations. Among the instrumental method for analysis, there is a renewed interest in UV Spectrophotometric methods due to methods being rapid, simple, and cost-effective compared to sophisticated HPLC, LC-MS/MS methods. Cardiovascular disease continues to occur in epidemic proportions globally. Hence multiple strategies are needed for control and reversal so as to reduce mortality and morbidity. A review of available literature indicated that ample analytical methods are available for the estimation of Metoprolol and Atorvastatin. These include HPTLC, Reverse phase HPLC methods for the drugs either individually or combined with other drugs. However, there is no reported UV-spectroscopic method for simultaneous estimation of the drugs based on the absorption correction principle. Hence, there is a need for a simple UV-spectroscopic method for the simultaneous analysis of cardiovascular drugs. A novel UV-spectroscopic method has been developed for the simultaneous estimation of cardiovascular drugs by the absorbance correction method. Methanol was used as a solvent, and water was used as a diluent for the analysis. The choice of wavelengths was based on the absorption correction principle. Accordingly, one wavelength was chosen where both drugs absorb, while the second wavelength was chosen such that only one drug was absorbed. The developed method was validated as per ICH guidelines. Linearity was obtained in the range of 10–100 µg/mL and 2–100 µg/mL for metoprolol tartrate and Atorvastatin calcium, respectively. The method was found to be accurate, precise, sensitive, and robust. The percentage assay value was found to be 99.10% for Metoprolol tartrate and 98.18% for Atorvastatin calcium which was within the acceptance criteria (90–110%). Hence, the developed UV method is a valuable, cost-effective tool for the quality control of drugs in analysis.