The aim here was to develop a specific, precise, accurate, linear, simple, rapid, validated, and cost-effective stability indicating UPLC-TUV method for the simultaneous estimation of Netarsudil and Latanoprost in bulk drug and ophthalmic preparations. The estimation was carried out on CHS C18 (2.6 x 50 mm, 1.6 µm) column, with the mobile phase composition of 60% 0.1N KH2PO4: 40% methanol. Flow rate: 0.3 mL/min. UV detection was carried out at 210 nm. The developed method was validated for linearity, accuracy, precision, the limit of detection, and quantification as per ICH guidelines. The linearity of the drugs from the concentration range of 5-30 µg/mL was established by constructing the calibration curve with a concentration on the X-axis and peak area on the Y-axis with the correlation coefficient of 0.999. The percentage recovery of drugs was found recovered within the range of 98.93-101.26%, and the mean of %recovered within the range of 99.93 -100.90%. Netarsudil and Latanoprost were eluted at 1.153 min and 1.480 min, respectively, with good resolution. Plate count and tailing factor were very satisfactory, so this method was optimized and to be validated. A new simple analytical method has been developed for the stability-indicating UPLC-TUV Method for the simultaneous estimation of Netarsudil and Latanoprost in bulk drug and ophthalmic preparations.
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