#125 Benzohydrazide schiff base derivatives: Design, synthesis, spectroscopic study and antimicrobial screening

Abstract

This research project aimed to design and synthesize hydrazide schiff base derivatives as an antimicrobial agent. Thus the objective was to perform in-silico studies of the designed compounds, synthesize, accomplish spectral analyses, and assess in-vitro antimicrobial activity. In-silico physicochemical properties using Molinspiration Cheminformatics software and computational toxicity studies with software, ProTox II was gaged for anticipated compounds. A four-step synthetic pathway was executed by both traditional condensation and microwave-assisted synthesis. Structural elucidation was conducted using Infrared spectroscopy (FTIR), Nuclear Magnetic Resonance (¹H & ¹³C-NMR), and mass spectroscopic studies of synthesized samples. Eventually, these derivatives were tested in-vitro against six bacterial strains (gram-positive: Bacillus subtilis, Staphylococcus aureus and gram-negative: Escherichia coli, Salmonella typhi, Klebsiella pneumonia, and Pseudomonas aeruginosa) with agar disc diffusion assay method and determined the area inhibited by these organisms (Zone of inhibition values). A total of fifteen of the twenty proposed compounds were synthesized, with ten derivatives moving forward for further research. Spectroscopic analyses such as IR, NMR, and mass spectroscopy aided in the structural elucidation of ten synthesized compounds, with their results reported in tabular form. Antimicrobial tests revealed that the compounds were highly effective against MTCC E. coli and S. typhi strains. Both of these microbes exposed substantial activity against IVb and IVj. E. coli and S. typhi were significantly inhibited by IVe and IVf, respectively. Conclusion: Consequently, substituted benzohydrazide Schiff bases were fruitfully designed, synthesized, and analyzed. In-vitro antimicrobial activity of the compounds indicated the potential of hydrazide Schiff bases.