#121 Synthesis of green nanofiber mat for tissue engineering applications

Abstract

Tissue engineering is the discipline that deals with the regeneration of damaged tissues. Nanofibers are the most acceptable substances for the regeneration of tissues because it mimics the extracellular cellular matrix (ECM), so the cells can quickly proliferate on it. Natural polysaccharides are used to develop nanofibers because of their biocompatibility and biodegradability. But certain limitations are also associated with these natural polysaccharides, such as low mechanical property, so chemically derived polymers are also incorporated with the natural polysaccharides to overcome this limitation. Nanofibers can be procured using various techniques; electrospinning is the most favored technique. This perspective aims to explore the combination of polyvinyl alcohol (PVA) and neem gum (NG) to fabricate nanofibers using the electrospinning technique. Various nanofibers were prepared by varying the concentration of PVA and NG. The preparation of nanofibers was confirmed using multiple analytical methods. The antimicrobial activity of PVA, NG, and PVA/NG nanofiber was investigated against bacteria such as Staphylococcus aureus and Escherichia coli using the disk diffusion method. Nutrient agar media was used for the incubation of bacteria. The NG and PVA/NG nanofiber can inhibit the growth of bacteria. This suggests that the nanofiber will provide a framework for the proliferation of cells and can treat the infections caused by bacteria.