The main aim of the present study is to investigate the effects of temperature and exposure time on the extraction of Opuntia ficus-indica and Opuntia dillenii cladodes. Cladodes and other parts of plants were rarely extracted for their constituents from literature. Screening, however, was not conducted to determine the influence of dependent variables on independent responses. The impact of the dependent variable on the response was determined using QbD software, namely Design Expert. A plot of Opuntia ficus-indica and Opuntia dillenii are poised, authenticated, and drenched with water to determine the effect of dependent variables (temperature and exposure time) on the response (% yield). The equation coded from this study for the percent yield was generated as +54.30+0.4061A-0.6432B+0.1500AB+0.8375A2+0.3875B2. For extraction of the contents of cladodes, 65 °C is the optimal temperature and exposure time is directly proportional.
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