Keywords
Nevirapine
Zidovudine
UV-Visible Spectrophotometry
simultaneous equation method
How to Cite
Abstract
A simple, accurate, precise, economical and reproducible UV-Visible Spectrophotometric method has been developed for the simultaneous estimation of Lamivudine, Nevirapine and Zidovudine in bulk and in combined tablet dosage form. The stock solutions were prepared in methanol followed by the further required dilutions with distilled water. This method involves the formation and solving of simultaneous equations at 271, 282 and 267 nm, as absorbance maxima of lamivudine, nevirapine and zidovudine, respectively. Beers law obeyed the concentration range of 1.5-9, 2.5-15 and 3-18 g/mL for Lamivudine, Nevirapine and Zidovudine, respectively. The results of analysis were validated statistically and by recovery studies. The %RSD for the recovery study was less than 2. The proposed method can be effectively applied for the simultaneous estimation of these drugs in bulk and in combined tablet dosage form.References
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