Keywords
metalazone
RP-HPLC
HPTLC
ICH guidelines
validation
How to Cite
Abstract
Two new, simple, accurate, and precise Reverse Phase High Performance Liquid Chromatograhic method and High Performance Thin Layer Chromatographic (HPTLC) method has been established for simultaneous analysis of Losartan Potassium and Metolazone in tablet formulations. The mobile consisting of Acetonitrile: 0.1% Trifluoroacetic acid, (40:60% v/v) at a flow rate of 0.8 mL/ min was used for RP-HPLC with a UV detector at 236 nm. For the HPTLC method, chloroform: Methanol, 10:1% v/v, as mobile phase, UV detection was performed densitometrically at 230 nm. The methods were validated in accordance with the requirements of ICH guidelines and USFDA guidelines. The methods were successfully used for determination of the drugs in tablets. Tablet excipients did not interfere with the chromatography.References
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